Sisters MairiAnne and Iona Mackenzie lost a Supreme Court case in June 2020, where they challenged the Victorian government's decision behind the Western highway's altered route between Beaufort and Ararat.

The new route required compulsory acquisition of some of the Mackenzie's land and the felling of several old trees, significant to the Indigenous community.

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The sisters appealed the decision in the Supreme Court of Appeals; however, Justices Tate, Kyrou and Kennedy rejected all 10 arguments.

They were ordered to pay an undisclosed amount.

A spokesperson from KORS Inc, a community group that supports the original route, said they were disappointed by the decision.

"This is a loss for all Victorians," they said.

"However, the decision proves how outdated the Environment Effects Act is."

A separate challenge by traditional owners is expected to return to court in the second half of this year.

The KORS spokesperson said they were also disappointed they are unable to meet with Louise Staley, state member for Ripon.

"KORS requested a meeting with Ms Staley to discuss relevant engineering and environmental information supporting safety upgrades and a viable alternative route, but were declined a meeting without explanation," they said.

"Ms Staley has continually called to "Get on with it" without offering any constructive solutions. We would welcome a constructive conversation with Ms Staley and her support for a review of the route."

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