Scott Morrison is "absolutely satisfied" none of his MPs were bullied because of their gender during the recent leadership spill.
A number of Liberal women had complained about bullying and intimidation as conservatives tried to oust Malcolm Turnbull.
But the prime minister says he's spoken directly to the MPs affected and he will deal with any issues they raise.
"During those periods they become moments of intense lobbying, and they become moments of a lot of pressure on all members," Mr Morrison told parliament on Wednesday.
"I'm absolutely satisfied there was no gender-specific lobbying or pressure placed on members."
West Australian senator Linda Reynolds hit out at bullying and intimidation in a Senate speech during the leadership spill, but now says the Liberal party must deal with the issue behind closed doors.
"Clearly there are issues we do need to address, and I'm now dealing with them internally in the party, in our processes the prime minister and the whips have set up," Senator Reynolds told Sky News.
The Liberal party is also facing attacks over the lack of women in its ranks, with men making up more than 75 per cent of its MPs.
Deputy Labor leader Tanya Plibersek said both major parties in 1994 had 14 per cent women - now Labor is close to 50 per cent while the Liberals are below 25 per cent.
Senator Reynolds said the Liberals don't need quotas for women, like Labor has used, but there are lessons to learn from the public service.
Ms Plibersek said she has engaged in "extreme conflict" over her 20 years in parliament, but Labor's culture is different because of the number of women.
"We make better decisions for the whole Australian community when we look more like the whole Australian community," she told the ABC.
South Australian Liberal senator Lucy Gichuhi threatened to use parliamentary privilege to name MPs accused of bullying and intimidation, but she backed down after talking with Mr Morrison.
Labor accused Mr Morrison of preparing to intervene in Senator Gichuhi's Liberal preselection battle in exchange for her backing down.
Mr Morrison conceded women are under-represented in the party but doesn't think quotas will help.
Former Liberal minister Craig Laundy backed gender quotas after the idea was first raised by colleague Sussan Ley.
Australian Associated Press